Disease: Burkitt's lymphoma Cell Type: B lymphocyte;
Cellular Products:
immunoglobulin (surface and secreted)
Permits/Forms:
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The cells are EBNA negative and are negative for Fc receptors.
Approximately 12% of the cells express commplement receptors.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Temperature: 37.0°C
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.
A saturation density of 2 X 10 exp6 is obtainable.
